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. 2020 Aug 11;3(10):e202000711. doi: 10.26508/lsa.202000711

Figure 4. MIC26 and MIC27 are dispensable for the spatial arrangement of MIC10 and MIC60 in mitochondria and the incorporation of other MICOS subunits into the complex.

Figure 4.

(A) Representative images of endogenous staining of MIC60 or MIC10 in HAP1 WT, MIC26 KO, MIC27 KO, or double knockout (DKO) cells using STED super-resolution nanoscopy show that the rail-like punctae arrangement of MIC60 or MIC10 remain unaltered in single knockouts or DKO cells. (B) Complexome profiling data representing the heat map of abundance of occurrence of MICOS subunits in isolated mitochondria from HAP1 WT, MIC26 KO, MIC27 KO, or DKO cells show that cluster of MICOS complex shifts to a lower molecular weight in DKO mitochondria but the remaining subunits remain associated to this complex. (C) Blue-native gel electrophoresis blotted for anti-MIC60 show MICOS complex in single knockouts or DKO cells lacking MIC26 and/or MIC27. BHM, bovine heart mitochondria.