Figure 6.
Cytoplasmic HTT aggregates accumulate more rapidly in the brains of R6/2(CAG)200 than in R6/2(CAG)90 mice. The pattern of immunostaining detected with the MW8 antibody on brain sections from R6/2(CAG)90 and R6/2(CAG)200 mice. (A) The hippocampal hilus of the dentate gyrus at 4 and 8 weeks of age. The location of the centre panel images in the brain sections is indicated in the adjacent thumbnails, and the position of the thumbnails within the tissue section is illustrated in Supplementary Figs 6 and 7. The wild type control sections are shown in Supplementary Figs 8 and 9. (B) The cortex, striatum, CA1 region of the hippocampus and hilus at 16 and 24 weeks of age for the R6/2(CAG)90 and 14 weeks of age for the R6/2(CAG)200 mice. The location of the images from within the tissue sections is illustrated in Supplementary Fig. 10. The wild type control sections are shown in Supplementary Fig. 11. n = 3/R6/2 genotype and n = 1/wild type control. (A) Centre panels, scale bar = 20 μm; thumbnails, scale bar = 200 μm. (B) scale bar = 20 μm.