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. 2020 Jul 16;12(14):14406–14417. doi: 10.18632/aging.103484

Figure 5.

Figure 5

osthole enhanced the cisplatin-induced apoptosis through the PTEN/AKT/Bad/Bcl-2 pathway in CD133+ HCC cells. (A) Effect of osthole (10 μmol/L) and PTEN siRNA on affecting the phosphorylation of AKT and Bad in CD133+ Huh7 and HepG2 cells. (B) Effect of osthole (10 μmol/L) and PTEN siRNA on affecting the interaction with Bad and Bcl-2 in CD133+ Huh7 and HepG2 cells. (C) Osthole (10 μmol/L) enhanced the effect of cisplatin (5 μmol/L) on reducing the mitochondrial membrane potential (ΔΨm) of CD133+ Huh7 and HepG2 cells. (D) Osthole (10 μmol/L) increased the cytosolic cytochrome c in CD133+ Huh7 and HepG2 cells which were treated with cisplatin (5 μmol/L). (E) Osthole (10 μmol/L) increased the apoptotic rate of CD133+ Huh7 and HepG2 cells which were treated with cisplatin (5 μmol/L).