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. 2020 Jul 27;12(14):14391–14405. doi: 10.18632/aging.103482

Figure 2.

Figure 2

DDR1 promotes EMT and glutamine metabolism in Hep3B cells. DDR1 plasmid or NC was transfected into Hep3B cells. The expression of DDR1 was determined by qRT-PCR (A) and western bolt (B) (n = 6, *p<0.05). (C) CKK-8 assay was used to examine the cell growth at 0, 24, 48 and 72 h (n = 10,*p<0.05). (D) Wound healing assay was used to detect cell migration (n = 6, *p<0.05). (E) Transwell assay was performed to check cell invasive ability (n = 6, *p<0.05). qRT-PCR (F) and western blot (G) were used to test the expression of EMT related genes: Vimentin, N-cadherin and E-cadherin (n = 6, *p<0.05). The protein expression of glutamine metabolism related genes: GLUD1, GLS1 and SLC1A was determined by western blot (H) and immunofluorescence (I) (n = 6, *p<0.05).