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. 2020 Jul 27;12(14):14391–14405. doi: 10.18632/aging.103482

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Copyright © 2020 Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Loss of DDR1 inhibits EMT and glutamine metabolism in SNU-182 cells. The siRNA of DDR1 or NC was transfected into SNU-182 cells. The expression of DDR1 was detected by qRT-PCR (A) and western blot (B) (n = 6, *p<0.05). (C) CKK-8 assay was used to examine cell growth at 0, 24, 48 and 72 h (n = 10, *p<0.05). (D) Wound healing assay was used to detect cell migration (n = 6, *p<0.05). (E) Transwell assay was performed to check cell invasion (n = 6, *p<0.05). qRT-PCR (F) and western blot (G) were used to test the expression of EMT related genes: Vimentin, N-cadherin and E-cadherin (n = 6, *p<0.05). (H) The protein expression of glutamine metabolism related genes: GLUD1, GLS1 and SLC1A was determined by western blot (n = 6, *p<0.05).