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. 2020 Aug 12;20:249. doi: 10.1186/s12906-020-03044-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — a Point-counting method to estimate the volume density of the cortex, medulla, cystic formation, and corpus luteum. The arrow indicates the right upper quadrant of each cross that was considered to be a point. b The optical disector method. An unbiased counting frame was laid on the images. The follicle oocytes were counted if they placed completely or partly inside the counting frame, touched the upper and right lines (acceptance line), or did not touch the left and lower borders (rejection lines) (arrows). Upper and lower guard zones were defined, and each of them was set to be 5 μm using a Heidenhain microcator (MT12, Germany)