Fig. 2.

Inhibition analysis of total fluorescent intensity/cell of lysotracker red by chloroquine, pyronaridine and artesunate in MCF7 Cells. Lysotracker accumulation in lysosomes is pH dependent, therefore a reduction in signal from the lysotracker suggests a pH increase in these organelles. This is proposed to be caused by accumulation of the charged base of the lysosomotropic compound in the lysosome, which in a lower pH environment becomes neutralized and trapped in the organelle. A) Representative images showing Lysotracker lysosomal accumulation inhibition at various concentrations. B) Graphical representation and quantification (Parentheses represent 95% CI) of the dose-dependent effect of on Lysotracker accumulation in lysosomes (Error bars represent SEM). Outliers were identified using the ROUT method (Q = 10%) and consequentially removed. C) Measure of cellular toxicity at concentrations and times mimicking the inhibition assays.