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. Author manuscript; available in PMC: 2020 Aug 13.
Published in final edited form as: Kidney Int. 2011 Jan 19;79(9):957–965. doi: 10.1038/ki.2010.534

Figure 3 |. X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) staining of kidneys of control and inv/inv mice carrying the BATlacZ reporter transgene.

Figure 3 |

(a) External appearance of X-gal staining of representative kidneys from control ( + / +, BATlacZ; lower) and inv (inv/inv, BATlacZ; upper) mice from 16.5 dpc through to 5 days of age. (b) The X-gal-positive staining of cortex and medulla in newborn control (lower) and inv (upper) kidneys. *cyst. (c) Quantitative analysis of BATlacZ reporter expression in inv and control kidney extracts. Transgene-specific β-galactosidase level was quantified by enzyme-linked immunosorbent assay. Control and inv kidneys all showed a similar level of BATlacZ expression. Each experimental group included at least three kidneys. 5d, 5-day-old; NB, newborn.