Table 2.
Primer Sequences for qPCR Analysis
| Genes | Sequence (5′- 3′) | Tm (°C) | Product Size |
|---|---|---|---|
| Creb1_F | GATCTTAGTGCCCAGCAACC | 58.62 | 175 bp |
| Creb1_R | TAGGCGAACTTCTCGCTTTC | 58.01 | |
| Creb2_F | GGGGCTGAAGAAAGCTTAGG | 58.24 | 329 bp |
| Creb2_R | GGGGGCTCCGTATTAGTCTC | 59.03 | |
| Creb3_F | CTCCAGGCCATGGTAATTGA | 57.27 | 97 bp |
| Creb3_R | CAGGTACAAAGAGGAGGCAGA | 59.10 | |
| Pparα_F | GGTGAGGACTTCGGCTTTTA | 57.53 | 110 bp |
| Pparα_R | AGTACTGCCGCTTCCACAAG | 60.32 | |
| Cpt1a_F | ATGTGGAAGTGGATGAAGTGC | 58.56 | 176 bp |
| Cpt1a_R | TTATCAGAGCCTTGGCGATT | 56.99 | |
| Rara_F | GTGAGGAACGACCGCAATAA | 58.28 | 247 bp |
| Rara_R | CGGTCTTGATGATGCACTTG | 57.18 | |
| Rarb_F | GCTACGAGATGACTGCTGAGT | 59.60 | 111 bp |
| Rarb_R | ATGCACTTGGTGGCCAGTTC | 61.18 | |
| Rarg_F | CGAGCTCAGAGGAGATGGTT | 58.89 | 215 bp |
| Rarg_R | CCTGGTCACCTTGTTGATGA | 57.43 | |
| Rxra_F | CTCTCCCATGAACCCTGTGA | 58.72 | 170 bp |
| Rxra_R | AGCTGTACACGCCATAGTGC | 60.46 | |
| Rxrb_F | GCTCCTTATCGCCTCCTTTT | 57.67 | 178 bp |
| Rxrb_R | CATCCTCATGTCACGCATTT | 56.50 | |
| Rxrg_F | GAATGACCTGGTCCTCCAAG | 57.59 | 205 bp |
| Rxrg_R | TGAAGACATGCCTGTGGAGA | 58.65 | |
| Hif1α_F | AGCTGCTGGAGACACAATCAT | 59.72 | 212 bp |
| Hif1α_R | CTTGATTGAGTGCAGGGTCA | 57.81 | |
| Bmp6 _F | AAGAAGGCTGGCTGGAATTT | 57.68 | 205 bp |
| Bmp6 _R | ACGTGTACCTCGCTCACCTT | 61.18 | |
| Gata4_F | AGCTTCATGTAGAGGCCACAG | 59.79 | 180 bp |
| Gata4_R | TGGCCTCTACCACAAGATGA | 58.05 | |
| Abca1_F | ACCCGCGTATTTTTCTCCAT | 57.59 | 121 bp |
| Abca1_R | GAGGGAGCATGTGGAGTTCT | 59.09 | |
| Pml_F | GTGCCCATCTATGCCTTCTC | 58.11 | 110 bp |
| Pml_R | ATGATCTTTCGGGAGCACTG | 57.68 | |
| Ptprn2_F | AAGCTTCGCACCAGAAAGTC | 58.77 | 80 bp |
| Ptprn2_R | GGCTCCAACCTCTACCACAT | 59.09 | |
| Chd4_F | AGGCATGTCCTACTGGCACT | 60.91 | 144 bp |
| Chd4_R | CGGCTCTTCTCTTCATCACC | 58.07 | |
| Lrpap1_F | AGGCTGACGACCTATACGACA | 60.41 | 188 bp |
| Lrpap1_R | CTCCAGCTCCTTCTTGGTGA | 59.02 | |
| Col1a1_F | GGTCCTGATGGCAAAACTG | 56.50 | 121 bp |
| Col1a1_R | CACCTTTAGGTCCAGGGAAT | 56.50 | |
| Col2a1_F | CTGTGACGAAGGGATTGTCCT | 59.72 | 287 bp |
| Col2a1_R | GGAGGTCCTTTGGGTCCTACA | 60.83 | |
| Col6a5_F | TGGCCACGTCTCAGTTTCAT | 59.60 | 424 bp |
| Col6a5_R | ACACGAGGTCAGCAAGTGAAT | 59.93 | |
| Col12a1_F | AGGCGAAAGGAAATCAGCCAC | 61.22 | 355 bp |
| Col12a1_R | AGCAGGGCATTTTGCTTCATC | 59.79 | |
| Acta1_F | GAAGGAGTAGCCACGCTCAG | 60.18 | 319 bp |
| Acta1_R | TGCTGTCCCTCTATGCCTCT | 60.03 | |
| TGF-β1_F | ATGAATAGCAGCCAGGTCAC | 57.66 | 95 bp |
| TGF-β1_R | GTGCTCACTGCTCCTGTGAT | 60.04 | |
| TGF-β2_F | ATCCCGCTTGAAATCAATGT | 55.69 | 206 bp |
| TGF-β2_R | AGACCCCACATCTCCTGCTA | 59.66 | |
| TGF-β3_F | GAGAGTTGCTCCACCTTTGG | 58.47 | 209 bp |
| TGF-β3_R | GTCCACGAACCTAAGGGCTA | 58.81 |