Table 1.
Potential HLA-I binding peptides identified in five non-structural proteins of SARS-CoV-2.
| Protein | Peptide ID | Peptide sequence | Position (star-end) | Percentile Rank | Viral antigenicity | Toxicity and Allergenicity | HLA-I interacting allele |
|---|---|---|---|---|---|---|---|
| NSP7 | P1 | LRVESSSKL | 20–28 | 1.60 | 0.60 | Negative | C*06:02 |
| P2 | LSMQGAVDI | 60–68 | 1.50 | 0.78 | Negative | C*01:02, C*02:02, C*04:01, C*12:02 | |
| P3 | EMLDNRATL | 74–82 | 0.17 | 0.63 | Negative | B*08:01, C*01:02, C*02:02, C*06:02, C*12:02, | |
| NSP8 | P4 | AAFATAQEA | 13–21 | 1.10 | 0.53 | Negative | C*12:02, C*02:02, |
| P5 | CVPLNIIPL | 114–122 | 0.87 | 1.37 | Negative | C*01:02 | |
| P6 | LAWPLIVTA | 180–188 | 0.38 | 1.02 | Negative | A*02:01, C*02:02, C*04:01, C*12:02, B*35:01 | |
| NSP9 | P7 | GLNNLNRGM | 93–102 | 1.80 | 1.01 | Negative | A*02:01, C*02:02, C*02:09, C*12:02 |
| P8 | NNLNRGMVL | 95–103 | 0.09 | 0.52 | Negative | B*08:01, C*06:02 | |
| P9 | GMVLGSLAA | 100–108 | 1.20 | 0.60 | Negative | A*02:01 | |
| P10 | VLGSLAATV | 102–100 | 0.20 | 0.66 | Negative | A*02:01, A*02:05, C*02:09, C*02:02 | |
| NSP12 | P11 | WEPEFYEAM | 116–124 | 0.21 | 0.53 | Negative | A*02:01 |
| P12 | MRNAGIVGV | 196–204 | 0.02 | 1.63 | Negative | C*06:02 | |
| P13 | LVYAADPAM | 372–380 | 0.02 | 0.52 | Negative | C*03:02, C*12:02, B*35:01, C*02:09, C*02:02, C*08:01 | |
| P14 | TVKPGNFNK | 409–417 | 0.02 | 1.38 | Negative | A*11:01 | |
| P15 | NAAISDYDY | 447–455 | 0.07 | 1.29 | Negative | B*35:01, A*01:01, C*03:03 | |
| P16 | FAYTKRNVI | 538–546 | 0.06 | 1.03 | Negative | B*08:01, C*03:02, C*12:02, C*08:01, C*02:02, C*02:09, C*01:02, C*06:02 | |
| P17 | IAATRGATV | 579–587 | 0.16 | 0.89 | Negative | B*08:01, C*01:02, C*02:02, C*03:02, C*02:09, C*08:01, C*12:02, | |
| P18 | ATVVIGTSK | 585–593 | 0.02 | 0.76 | Negative | A*11:01 | |
| P19 | RLANECAQV | 654–662 | 0.16 | 0.93 | Negative | A*02:01 | |
| P20 | MLDMYSVML | 899–907 | 0.08 | 0.56 | Negative | A*02:01, A*01:01, C*01:02, C*08:01, | |
| NSP13 | P21 | HVISTSHKL | 33–41 | 0.10 | 0.69 | Negative | C*01:02, C*02:02, C*03:02, C*12:02, B*35:01, |
| P22 | TEETFKLSY | 141–149 | 0.65 | 0.65 | Negative | A*01:01 | |
| P23 | YGIATVREV | 149–157 | 0.37 | 1.43 | Negative | C*02:02, C*03:02, C*12:02, | |
| P24 | PTLVPQEHY | 238–246 | 0.43 | 0.78 | Negative | A*01:01 | |
| P25 | TLVPQEHYV | 239–247 | 0.04 | 0.54 | Negative | A*02:01, C*02:02 | |
| P26 | EHYVRITGL | 244–252 | 0.33 | 0.57 | Negative | B*08:01 | |
| P27 | SHFAIGLAL | 289–297 | 0.35 | 1.39 | Negative | C*02:02, C*03:02, C*12:02, B*40:01, | |
| P28 | VVFDEISMA | 371–379 | 0.11 | 0.74 | Negative | A*02:01, A*02:05, C*02:02, C*12:02 | |
| P29 | VVNARLRAK | 386–394 | 0.08 | 1.93 | Negative | A*03:01, A*11:01 | |
| P30 | EIVDTVSAL | 447–455 | 0.26 | 0.66 | Negative | C*02:02, C*03:02, C*04:01, C*12:02, B*35:01 | |
| P31 | ITRAKVGIL | 565–573 | 0.25 | 0.91 | Negative | B*08:01 | |