Table 2.
Potential HLA-II binding peptides identified in five non-structural proteins of SARS-CoV-2.
| Protein | Peptide ID | Peptide sequence | Position (star-end) | Percentile Rank | Viral antigenicity | Toxicity and Allergenicity | IFN-g induction | HLA-I interacting allele |
|---|---|---|---|---|---|---|---|---|
| NSP7 | P32 | VVLLSVLQQLRVESS | 11–25 | 0.60 | 0.50 | Negative | Positive | DRB1*01:01, DRB1*01:02, DRB1*03:01, DRB1*03:07, DRB1*04:01, DRB1*04:02, DRB1*04:04, |
| NSP8 | P33 | IASEFSSLPSYAAFA | 2–16 | 0.13 | 0.54 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 |
| P34 | SEFSSLPSYAAFATA | 4–18 | 0.15 | 0.54 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 | |
| P35 | FSSLPSYAAFATAQE | 6–20 | 7.20 | 0.51 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 | |
| P36 | GCVPLNIIPLTTAAK | 113–127 | 3.00 | 1.12 | Negative | Positive | DRB1*03:06, DRB1*03:07, DRB1*04:04, DRB1*04:01 | |
| P37 | PLNIIPLTTAAKLMV | 116–130 | 6.10 | 0.82 | Negative | Positive | DRB1*03:06, DRB1*03:07, DRB1*04:04, DRB1*04:01 | |
| P38 | WEIQQVVDADSKIVQ | 154–168 | 7.60 | 0.53 | Negative | Positive | DRB1*03:01, DRB1*03:06, DRB1*03:07 | |
| P39 | IQQVVDADSKIVQLS | 156–170 | 7.20 | 0.59 | Negative | Positive | DRB1*03:01, DRB1*03:06, DRB1*03:07 | |
| P40 | PNLAWPLIVTALRAN | 178–192 | 8.50 | 0.92 | Negative | Positive | DRB1*01:01 | |
| P41 | NLAWPLIVTALRANS | 179–193 | 3.50 | 0.99 | Negative | Positive | DRB1*03:06, DRB1*03:07, DRB1*04:04 | |
| P42 | LAWPLIVTALRANSA | 180–194 | 3.10 | 0.80 | Negative | Positive | DRB1*01:01, DRB1*03:06, DRB1*03:07, DRB1*04:04 | |
| P43 | AWPLIVTALRANSAV | 181–195 | 3.30 | 0.72 | Negative | Positive | DRB1*01:01, DRB1*03:06, DRB1*03:07, DRB1*04:04 | |
| P44 | WPLIVTALRANSAVK | 182–196 | 2.60 | 0.74 | Negative | Positive | DRB1*01:01, DRB1*03:06, DRB1*03:07, DRB1*04:04 | |
| P45 | PLIVTALRANSAVKL | 183–197 | 0.45 | 0.66 | Negative | Positive | DRB1*01:01, DRB1*01:02, DRB1*03:01, DRB1*03:06, DRB1*03:07, DRB1*04:01, DRB1*04:04 | |
| NSP9 | P46 | GPKVKYLYFIKGLNN | 82–96 | 7.20 | 0.70 | Negative | Positive | DRB1*04:04 |
| P47 | PKVKYLYFIKGLNNL | 83–97 | 6.10 | 0.62 | Negative | Positive | DRB1*01:01, DRB1*04:04 | |
| P48 | KVKYLYFIKGLNNLN | 84–98 | 4.40 | 0.91 | Negative | Positive | DRB1*04:01, DRB1*01:01, DRB1*04:04 | |
| P49 | KYLYFIKGLNNLNRG | 86–100 | 0.38 | 0.64 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 | |
| P50 | YLYFIKGLNNLNRGM | 87–101 | 0.31 | 0.52 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 | |
| P51 | LYFIKGLNNLNRGMV | 88–102 | 0.31 | 0.52 | Negative | Positive | DRB1*01:01, DRB1*04:01, DRB1*04:04 | |
| P52 | NNLNRGMVLGSLAAT | 95–109 | 1.20 | 0.82 | Negative | Positive | DRB1*03:06, DRB1*03:07, DRB1*04:02 | |
| P53 | NLNRGMVLGSLAATV | 96–110 | 1.20 | 0.83 | Negative | Positive | DRB1*03:06, DRB1*03:07, DRB1*04:02 | |
| P54 | LNRGMVLGSLAATVR | 97–111 | 1.20 | 0.92 | Negative | Positive | DRB1*04:04 | |
| NSP12 | P55 | NAGIVGVLTLDNQDL | 198–212 | 1.70 | 1.30 | Negative | Positive | DRB1*04:05, DRB1*04:03 |
| P56 | RLSFKELLVYAADPA | 365–379 | 3.50 | 0.59 | Negative | Positive | DRB1*04:01, DRB1*04:03 DRB1*01:01 |
|
| P57 | LSFKELLVYAADPAM | 366–380 | 3.00 | 0.65 | Negative | Positive | DRB1*04:01 DRB1*04:03, DRB1*01:01 DRB1*04:05 |
|
| P58 | AASGNLLLDKRTTCF | 382–396 | 2.40 | 0.89 | Negative | Positive | DRB1*03:01 | |
| P59 | ASGNLLLDKRTTCFS | 383–397 | 1.70 | 0.94 | Negative | Positive | DRB1*03:01 | |
| P60 | SGNLLLDKRTTCFSV | 384–398 | 1.40 | 1.04 | Negative | Positive | DRB1*03:01 | |
| P61 | GNLLLDKRTTCFSVA | 385–399 | 1.90 | 1.20 | Negative | Positive | DRB1*03:01 | |
| P62 | NLLLDKRTTCFSVAA | 386–400 | 3.80 | 1.20 | Negative | Positive | DRB1*03:01 | |
| P63 | KHFFFAQDGNAAISD | 438–452 | 1.10 | 0.54 | Negative | Positive | DRB1*04:01, DRB1*01:03 DRB1*04:03, DRB1*01:01 |
|
| P64 | KRNVIPTITQMNLKY | 532–546 | 0.96 | 1.25 | Negative | Positive | DRB1*04:03 | |
| P65 | ITQMNLKYAISAKNR | 539–553 | 1.20 | 1.51 | Negative | Positive | DRB1*04:03, DRB1*01:03 DRB1*04:01 |
|
| P66 | QMNLKYAISAKNRAR | 541–555 | 0.32 | 1.50 | Negative | Positive | DRB1*04:03, DRB1*01:03, DRB1*04:01, DRB1*04:05 | |
| P67 | MNLKYAISAKNRART | 542–556 | 0.44 | 1.44 | Negative | Positive | DRB1*04:03, DRB1*04:01 DRB1*01:03 |
|
| P68 | NLKYAISAKNRARTV | 543–557 | 0.96 | 1.34 | Negative | Positive | DRB1*04:03, DRB1*01:03 DRB1*04:01 |
|
| P69 | MLRIMASLVLARKHT | 629–643 | 0.43 | 0.53 | Negative | Positive | DRB1*04:03, DRB1*03:01 DRB1*01:01, DRB1*01:03 DRB1*04:01, DRB1*04:05 |
|
| P70 | LRIMASLVLARKHTT | 630–644 | 0.53 | 0.67 | Negative | Positive | DRB1*03:01, DRB1*04:03 DRB1*01:01, DRB1*01:03, DRB1*04:01 |
|
| P71 | ASQGLVASIKNFKSV | 771–785 | 0.85 | 0.58 | Negative | Positive | DRB1*04:03, DRB1*04:01 DRB1*03:01 |
|
| NSP13 | P72 | EVLSDRELHLSWEVG | 156–170 | 2.90 | 1.54 | Negative | Positive | DRB1*03:01 |
| P73 | TTYKLNVGDYFVLTS | 215–229 | 2.50 | 0.60 | Negative | Positive | DRB1*03:02 | |
| P74 | LNVGDYFVLTSHTVM | 219–233 | 3.00 | 0.61 | Negative | Positive | DRB1*01:01, DRB1*01:03 | |
| P75 | DYFVLTSHTVMPLSA | 223–237 | 0.96 | 0.56 | Negative | Positive | DRB1*01:01, DRB1*01:03 | |
| P76 | RFNVAITRAKVGILC | 560–574 | 3.60 | 1.32 | Negative | Positive | DRB1*04:02, DRB1*01:03 | |
| P77 | KVGILCIMSDRDLYD | 569–583 | 0.98 | 1.51 | Negative | Positive | DRB1*03:01, DRB1*03:02 | |