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. 2020 Jul 13;61(8):15. doi: 10.1167/iovs.61.8.15

Figure 3.

Figure 3.

Accumulation of autofluorescent granules in the RPE in GA borders of donors with AMD. Representative bright field images overlaid with epifluorescence image in the green-red (A, C, E, G, I, K) and far-red (B, D, F, H, J, L) channels. Human cryosections of a matched control (A, B) and AMD donor eyes at the GA border (C–L) were observed on epifluorescence in the green-red and far-red channels; autofluorescence was merged into bright field images. The RPE from a control eye showed the prevalence of granules fluorescent in the green-red (A) and far-red (B) channels and a several dark-pigment non-fluorescent granules aligned along the apical surface (white arrowheads). The AMD donor eyes at the GA border (C–L) displayed hypertrophic RPE cells with their cytoplasm filled with granules fluorescent both in the green-red and far-red channels; a few dark-pigment nonfluorescent granules are present and distributed through the cytoplasm. Ch, choroid. Scale bar: 100 µm. * Bruch's membrane.