Table 6.
Retinal Thickness (in µm) of OD and OS Eyes: MINITAB Output of the Repeated Measures Experiment with Three Factors: Treatment Group, Eye, and Quadrant*
| OS | OD | |||||||
|---|---|---|---|---|---|---|---|---|
| Design | Q1 | Q2 | Q3 | Q4 | Q1 | Q2 | Q3 | Q4 |
| Control | G1 | G1 | G1 | G1 | G1 | G1 | G1 | G1 |
| EAE | G2 | G2 | G2 | G2 | G2 | G2 | G2 | G2 |
| EAE + treatment | G3 | G3 | G3 | G3 | G3 | G3 | G3 | G3 |
| Degrees of | Adjusted Sum | Adjusted Mean | ||||||
| ANOVA Table | Freedom | of Squares | Square | F | P | |||
| Between subject | ||||||||
| Treatment | 2 | 2297.86 | 1148.93 | 12.25 | 0.000 | |||
| Subject | 30 | 2814.15 | 93.80 | 7.41 | 0.000 | |||
| Within subject | ||||||||
| Eye | 1 | 5.40 | 5.40 | 0.43 | 0.514 | |||
| Treatment × eye | 2 | 2.38 | 1.19 | 0.09 | 0.910 | |||
| Quadrant | 3 | 36.97 | 12.32 | 0.97 | 0.406 | |||
| Treatment × quadrant | 6 | 68.45 | 11.41 | 0.90 | 0.495 | |||
| Eye × quadrant | 3 | 17.51 | 5.84 | 0.46 | 0.710 | |||
| Treatment × eye × quadrant | 6 | 92.61 | 15.43 | 1.22 | 0.298 | |||
| Residual | 210 | 2659.15 | 12.66 | — | — | |||
| Total | 263 | 8001.26 | — | — | — | |||
The residual sum of squares pools the interaction sums of squares between subjects and the effects of eye, quadrant, and eye by quadrant interaction. The three-factor ANOVA in GraphPad Prism8 is quite limited (two of the three factors can only have two levels) and could not be used.