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. 2020 Jul 29;2(8):e485–e496. doi: 10.1016/S2665-9913(20)30168-5

Figure 4.

Figure 4

Top hits from BRF model validated with logistic regression analysis and sPLS-DA

(A) Odds ratios (error bars indicate 95% CIs) of 28 immunological parameters were computed with univaraite logistic regression analysis. iNKT and PDC data is shown seperately inset because of very different CI values. (B) sPLS-DA model optimisation using ten-fold cross-validation. (C) sPLS-DA plot to validate the top hits from the predictive model. Individual distribution points and confidence ellipses (ovals) are plotted for the healthy control and juvenile-onset SLE groups. (D) Using this analysis, the weighting of each cell type in component 1 and 2 is displayed (inner circle is the 0·5 cutoff). (E) Factor loading weights in component 1 for the top ten ranked immunological parameters. The bars indicate the class with maximal mean value. Variables excluded from the plot have no weight in component 1. BRF=balanced random forest. CM=central memory. EM=effector memory. EMRA=effector memory cells re-expressing CD45RA. iNKT=invariant natural killer T cells. PDCs=plasmacytoid dendritic cells. SLE=systemic lupus erythematosus. sPLS-DA=sparse partial least squares-discriminant analysis. Treg=regulatory T cells. Tresp=responder T cells.