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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Nat Cell Biol. 2020 Jul 27;22(8):960–972. doi: 10.1038/s41556-020-0550-8

Extended Data Fig. 5. AKAP95 partially localizes in nuclear speckles and with actively transcribing Pol II.

Extended Data Fig. 5

Fluorescence microscopy images of HeLa cells transiently expressing AKAP95-GFP. Nucleus DNA was stained by DAPI, and specific proteins were stained with antibodies for SRSF2 (a), Pol II (b), and Pol II-S2P (c). The assays were repeated 10 times for each staining and show similar trend. Right, quantification of the signal intensity of indicated molecules across the dotted lines shown in the images. Quantification by Image J. Scale bar, 5 μm for all. Statistical source data are provided as in source data extended data fig 5.