Table 1). Ceramide, HexCer, and SM species from WT and Sphk1 KO mouse retinas.

Retinas were analyzed by LC/MS for individual species of ceramides, HexCer, and sphingomyelin; pmol/mg wet weight tissue.

Sphingolipid Species	Ceramides (pmol/mg tissue)		Monohexosylceramides (pmol/mg tissue)		Sphingomyelin (pmol/mg tissue)
	WT	KO	WT	KO	WT	KO
14:0	1.4 ± 0.28	2.9 ± 0.69	0.30 ± 0.00*	0.50 ± 0.070*	7.0 ± 1.2	9.0 ± 0.71
16:0	68 ± 11	150 ± 34	1.9 ± 0.13*	4.4 ± 0.75*	120 ± 12	170 ± 23
18:1	2.2 ± 0.41	6.4 ± 1.8	0.03 ± 0.030*	0.10 ± 0.00*	25 ± 4.1*	57 ± 8.1*
18:0	79 ± 5.1*	160 ± 31*	6.0 ± 1.8	13 ± 5.8	93 ± 6.7	170 ± 40
20:0	27 ± 8.1	59 ± 14	2.3 ± 0.70	3.8 ± 1.2	75 ± 11	140 ± 30
22:0	16 ± 3.4*	34 ± 5.7*	7.3 ± 3.9	11 ± 6.6	59 ± 4.6*	96 ± 14*
24:1	21 ± 1.7*	44 ± 8.5*	12 ± 7.4	26 ± 19	37 ± 5.1	62 ± 13
24:0	6.6 ± 0.56*	16 ± 3.7*	11 ± 6.0	19 ± 11	21 ± 3.5	37 ± 8.8
26:1	0.25 ± 0.90	0.40 ± 0.11	0.45 ± 0.25	0.68 ± 0.38	0.65 ± 0.090*	1.4 ± 0.27*
26:0	1.4 ± 0.88	1.8 ± 0.72	0.20 ± 0.070	0.18 ± 0.050	0.45 ± 0.030	0.75 ± 0.13
Total	220 ± 30*	480 ± 91*	41 ± 20	79 ± 43	440 ± 26*	740 ± 96*

Data presented as mean ± SEM, two-tailed Student’s t-test n=4,

^*p= >0.05-0.01.