Fig 1. Eyeblink serial feature-positive discrimination task in mice.

(A) The schematic time sequence of the cue, the conditioned stimulus (CS), and the unconditioned stimulus (US). Thirty cued and non-cued trials were randomly performed in a daily session with intertrial intervals between 60 and 70 s. In a cued trial, a 2-s light cue was delivered randomly 5 or 6 s before a 350-ms tone CS, which co-terminated with a 100-ms periorbital electrical shock US. In a non-cued trial, the CS was presented alone without the preceding cue or the US. (B) Examples of integrated EMG of a mouse averaged over the cued (solid line) and non-cued trials (dotted line) in a session. The horizontal axis represents the time course (ms) and the vertical axis illustrates the magnitude of the EMG response (%).