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. 2020 Aug 13;15(8):e0237442. doi: 10.1371/journal.pone.0237442

Fig 4. SB203580 reduces while IL-6 promotes PLum-AD and PLum-AI migratory potential in a time-dependent manner.

Fig 4

(A) Representative bright-field images showing effect of treatment with SB203580 (10 μM) and IL-6 (10 ng/mL) on cell migration. Scale = 100μm. A scratch was made in a 12-well plate of confluent PLum-AD or Plum-AI cells using a 200 μL tip, and images were taken at T = 0 and 48 hours. (B) Quantification of the distance of wound closure (wound distance in %) was assessed over time and represented by the line graphs. (C) Trans-well migration assay. PLum-AD and PLum-AI cells were plated onto 8 μm pore size inserts placed in 24-well plates containing media with or without treatment with SB203580 or IL-6. Media containing 5% FBS was used in the lower chamber as chemoattractant. Quantification of the migrated cells was assessed by taking the average of migrating cells counted in 6 randomly selected fields for every condition at 10x magnification of an inverted light microscope. Data represents an average of three independent experiments and are reported as mean ± SEM (One-way ANOVA with Bonferroni’s corrected test for multiple comparisons of each treatment with the control *P < 0.05; **P < 0.01; ***P < 0.001).