Fig 3.

(A) Constitutive activation of p38 was obliterated in SRA-deficient B16 cells. The expression of total and phosphorylated p38 as well as cleaved Notch-1, were measured by western blot. Three repeated experiments with one representative blot. (B) Inhibition of p38 in control B16 cells (B16-shCtrl) enhanced pigmentations. SB202190, a p38 inhibitor, at 10 μM, was incubated with B16 cells for 24 h. Melanin concentration was then measured (n = 3 and 3, respectively, for B16-shCtrl cells and B16-SRAi cells; error bars represent standard errors; * indicates p<0.05).