Fig 4.

(A) RNA interference against SRA in human amelanotic A375 melanoma cells induced TRP1 and TRP2 expression. A375 cells were transfected with either siRNA against SRA (siSRA) or control sequence (siCtrl). The expression of MITF, TYR, TRP1, and TRP2 was measured by western blot. Three repeated experiments with one representative blot. (B) The A375 cells, including A375 non-transfected, A375-siSRA, and A375-siCtrl, were processed for real-time PCR to measure the expression of SRA, MITF, TYR, TRP1, and TRP2 (n = 3 and 3, respectively; error bars represent standard errors; * indicates p<0.05). (C)The expression and phosphorylation of p38 were measured in A375-siSRA and A375-siCtrl cells by western blot. To address the role of p38 in SRA activation in A375 cells, cells with or without SRA knockdown were also treated with SB202190, a p38 inhibitor, for 24 h at 10 μM. Three repeated experiments with one representative blot.