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. 2020 Aug 13;15(8):e0234492. doi: 10.1371/journal.pone.0234492

Fig 5. The apoptosis rate of HUVECs was measured using TUNEL assay.

Fig 5

The figure showed a representative set of the graph of apoptosis rate of HUVECs by TUNEL staining. A: the indicated concentrations of sFlt-1-treated group, and B: statistical result of apoptosis rate of HUVECs in different groups. TUNEL analysis showed that sFlt-1 could induce an abundance of apoptosis in a dose-dependent manner (100 ng/ml vs. 0 ng/ml: 22.0 ± 3.60% vs. 5.67 ± 2.52%, p = 0.003; 1000 ng/ml vs. 0 ng/ml: 34.67 ± 6.03% vs. 5.67 ± 2.52%, p = 0.002; 1000 ng/ml vs. 100 ng/ml: 34.67 ± 6.03% vs. 22.0 ± 3.60%, p = 0.035). Results are the means ± standard deviation of three independent experiments. Scale bar denotes 50μm. Arrows indicate TUNEL-positive cells.