Table 3. Top 15 genes with functional annotations ranked by Hepatocystis sp. dN in comparison of Hepatocystis sp., P. berghei ANKA and P. ovale curtisi.
Genes with completely unknown function and genes with very little information on their possible functions have been left out from this table. The rank column indicates the Hepatocystis sp. dN rank of each gene in the complete table (with 4009 genes) that includes genes with unknown function (S4 Table).
| Gene id |
Hepato- cystis dN |
P. berghei dN | P. ovale dN | Annotations | Rank | Putative function |
|---|---|---|---|---|---|---|
| HEP_00146800 PBANKA_1303400 PocGH01_12075300 |
1.08 | 0.21 | 0.42 | Oocyst rupture protein 2 (ORP2) | 3 | Sporozoite egress from the oocyst [32] |
| HEP_00446500 PBANKA_1003000 PocGH01_03012800 |
0.71 | 0.19 | 0.34 | liver specific protein 2 (LISP2) | 19 | Involved in liver stage development [33] |
| HEP_00295100 PBANKA_0905900 PocGH01_09049300 |
0.71 | 0.31 | 0.63 | AP2-O | 20 | Essential for morphogenesis in ookinete stage in Plasmodium [34] |
| HEP_00035800 PBANKA_1107600 PocGH01_10033500 |
0.69 | 0.27 | 0.16 | 6-cysteine protein (p38) | 24 | P. berghei p38 is expressed in gametocytes and in asexual blood stages [28] |
| HEP_00213800 PBANKA_1001800 PocGH01_03011500 |
0.67 | 0.45 | 0.63 | AP2 domain transcription factor AP2-SP2 | 28 | Required for sporozoite production in Plasmodium [35] |
| HEP_00337100 PBANKA_0112100 PocGH01_11043100 |
0.62 | 0.36 | 0.42 | AP2 domain transcription factor ApiAP2 | 40 | Involved in blood stage replication [35,36] |
| HEP_00456700 PBANKA_0512800 PocGH01_06021700 |
0.62 | 0.61 | 0.38 | Merozoite TRAP-like protein (MTRAP) | 42 | Essential for gamete egress from erythrocytes [29] |
| HEP_00304800 PBANKA_1353400 PocGH01_12023100 |
0.62 | 0.32 | 0.26 | Secreted ookinete protein (PSOP7) | 43 | Secreted ookinete proteins are necessary for invasion of the mosquito midgut [37] |
| HEP_00254800 PBANKA_1449000 PocGH01_14060000 |
0.59 | 0.26 | 0.26 | Microgamete surface protein MiGS | 54 | Plays a critical role in male gametocyte osmiophilic body formation and exflagellation [30] |
| HEP_00115700 PBANKA_0304400 PocGH01_04023000 |
0.55 | 0.41 | 0.15 | Merozoite surface protein 4 (MSP4) | 62 | Merozoite surface proteins are involved in red blood cell invasion [38] |
| HEP_00166600 PBANKA_0301000 PocGH01_04026800 |
0.54 | 0.36 | 0.2 | Repetitive organellar protein (ROPE) | 64 | Localised to the apical end of merozoites, possibly involved in red blood cell invasion [39] |
| HEP_00195400 PBANKA_1463000 PocGH01_14074600 |
0.54 | 0.24 | 0.26 | Osmiophilic body protein (G377) | 67 | Female‐specific protein, affects the size of the osmiophilic body and female gamete egress efficiency [31] |
| HEP_00130400 PBANKA_1358000 PocGH01_12018000 |
0.53 | 0.17 | 0.11 | Thioredoxin 2 (TRX2) | 75 | Part of a protein complex in parasitophorous vacuolar membrane, required for pathogenic protein secretion into host [40], important for maintaining normal blood‐stage growth [41] |
| HEP_00391300 PBANKA_0313400 PocGH01_04013500 |
0.52 | 0.49 | 0.27 | Autophagy-related protein 11 (ATG11) | 76 | Predicted to be involved in cargo selection in selective autophagy [42] |
| HEP_00155500 PBANKA_1302300 PocGH01_12076400 |
0.52 | 0.18 | 0.17 | Metacaspase-2 | 80 | Protease with caspase-like activity [43] |