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. 2020 Jul 30;9:e55474. doi: 10.7554/eLife.55474

Figure 2. Single-cell decomposition of the epididymis.

(A) Single-cell cluster (expanded from Figure 1C) with 21 clusters annotated according to predicted cell types. Inset shows coarser cell type groupings used for downstream subclustering. (B) Heatmap of genes enriched in each cluster. Among the genes exhibiting significant variation across the 21 clusters, 1241 genes enriched at least fourfold in at least one cluster were selected for visualization. Heatmap shows these genes sorted according to the cluster where they exhibit maximal expression. (C) Expression of key marker genes across the entire dataset. See also Figure 2—figure supplement 1. For this and other single-cell images, color scale runs from gray (no expression) through blue, then red and finally orange (highly expressed), with heatmap for each panel scaled according to expression level of the gene in question.

Figure 2.

Figure 2—figure supplement 1. Additional images of gene expression levels across the entire single-cell dataset.

Figure 2—figure supplement 1.

(A) Gpx genes, including classic markers of caput (Gpx5) and cauda (Gpx3) principal cells, shown as in Figure 2C. (B) Several well-known sperm mRNAs, shown as in (A). We find no evidence for any sperm being captured, consistent both with our efforts to remove as many sperm as possible, and with the expectation that the unusually elongated dimensions of sperm would be incompatible with the droplet sizes used in the 10X Chromium platform.