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. 2020 Aug 13;9:e57936. doi: 10.7554/eLife.57936

Figure 5. The Rec12 protein does not promote fertility in heterozygous S. pombe diploids.

Figure 5.

(A) Schematic of viable spores resulting from an ‘inbreeding’ scenario. The three indicated types of spores are viable when wtf drivers are homozygous. (B) Schematic of viable spores resulting from an ‘outcrossing’ scenario where one set of wtf drivers is heterozygous. Haploid spores that only inherit one wtf allele will be killed by the poison of the wtf they did not inherit. Spores that inherit both wtf drivers due to a wtf duplication or disomy (aneuploidy or diploidy) will survive. Other outcomes of meiosis are not represented in this figure. (C) Viable spore yield values of heterozygous and homozygous S. pombe diploids in rec12+ and rec12Δ backgrounds. * indicates p-value<0.05 (Wilcoxon test) when comparing the rec12+ to rec12Δ fertility values. We compared the viable spore yield of each diploid in rec12+ and rec12Δ backgrounds. At least three, but usually more independent diploids were used to calculate viable spore yield. The data for rec12+ diploids is repeated from Figure 1. The data for Sk/Sk rec12Δ diploid (diploid 19) is repeated from Figure 3. The raw data are reported in Figure 5—source data 1.

Figure 5—source data 1. Raw data of the viable spore yield reported in Figure 5.
The top columns represent the S. pombe diploid assayed and the backgrounds in which it was tested (rec12+ or rec12Δ). The SZY strain numbers of both of the haploid parental strain are shown underneath the rec12 genotype. We present all of the viable spore yield values from independent assays. For statistical analyses, in every case, the rec12Δ diploid was compared to the rec12+ diploid. The raw data from the rec12+ crosses are also shown in Figure 1—source data 1. The raw data for the Sk rec12Δ are also presented in Figure 3—source data 2.