DSS-3 can kill in suspension using a diffusible, density-dependent mechanism. Cell density (CFU/ml) of DSS-3 Kn (black) and TM1035 (gray) in liquid coculture. (A) All 9 replicates for the undiluted liquid competition assay. (B) A representative experiment for the liquid dilution experiment. Asterisks denote time points where TM1035 CFU/ml are statistically lower than that of the previous time point (P < 0.05, Student’s t test). (C) Percent recovery of tagged TM1035 at 24 h when grown on a filter above an empty control, a differentially tagged TM1035 strain, DSS-3, or 2 μg kanamycin antibiotic. Asterisks denote P < 0.0001 using a Student t test comparing each experimental condition to the TM1035/empty control. Error bars indicate standard error, although some are too small to be seen.