TABLE 1.
Cell wall-associated proteins found in A. fumigatus EVs produced by protoplastsa
| Condition | UniProt annotation | UniProt no. |
Accession no. | Standard name(s) |
|---|---|---|---|---|
| Cell wall synthesis only | Probable glucan endo-1,3-beta-glucosidase EglC | B0XXF8 | AFUB_048180 | Bgt2 |
| Probable beta-glucosidase E BglE | B0YD91 | AFUB_094720 | Exg21 | |
| Filament-forming protein (Tpr/p270), putative | B0XM26 | AFUB_001640 | ||
| Cell wall biogenesis protein Mhp1, putative | B0XR76 | AFUB_012380 | ||
| O-Methyltransferase | B0XVZ1 | AFUB_033500 | ||
| 1,4-Alpha-glucan branching enzyme | B0Y0Q4 | AFUB_058160 | ||
| Alpha-1,2-mannosyltransferase (Alg2), putative | B0Y1U9 | AFUB_060920 | Alg2 | |
| Alpha-1,6-mannosyltransferase subunit (Och1), putative | B0Y410 | AFUB_056120 | Och1 | |
| Glycan biosynthesis protein (PigL), putative | B0YAG1 | AFUB_084550 | PigL | |
| Alpha-1,2-Mannosidase | B0Y765 | AFUB_072720 | ||
| Alpha-1,2-mannosyltransferase, putative | B0Y1T7 | AFUB_060800 | MnnII | |
| Alpha-N-acetylglucosamine transferase | B0YA98 | AFUB_083900 | ||
| Cell wall repression only | Mannosylphosphorylation protein (Mnn4), putative | B0XN98 | AFUB_004200 | Mnn4 |
| GPI-anchored cell surface glycoprotein, putative | B0Y1D8 | AFUB_059930 | ||
| Chitin synthase activator (Chs3), putative | B0Y9Q8 | AFUB_081930 | Chs3 | |
| Mannosyltransferase PMTI | B0YA13 | AFUB_083000 | Pmt4 | |
| Probable glucan endo-1,3-beta-glucosidase BtgC | B0Y429 | AFUB_056310 | Bgt3 | |
| Cell wall proline-rich protein, putative | B0XRJ9 | AFUB_012940 | ||
| Glycosyl hydrolase, putative | B0XYB1 | AFUB_040280 | ||
| Both cell wall synthesis and cell wall repression |
Chitin synthase, putative | B0XTD9 | AFUB_029070 | CsmB |
| Chitin synthase ChsE | B0XTE0 | AFUB_029080 | ChsE | |
| Chitin synthase | B0XTK9 | AFUB_018960 | ChsA | |
| Chitin synthase activator (Chs3), putative | B0XZ75 | AFUB_043410 | Chs3 | |
| Chitin synthase | B0XZY5 | AFUB_034810 | ChsG | |
| Chitin synthase F | B0Y9Q7 | AFUB_081920 | ChsF | |
| Class V chitinase, putative | B0YBH2 | AFUB_092800 | ||
| Chitin biosynthesis protein (Chs5), putative | B0YDJ8 | AFUB_095840 | Chs5 | |
| Chitin biosynthesis protein (Chs7), putative | B0XQX5 | AFUB_011500 | Chs7 | |
| Alpha-1,3-glucan synthase, putative | B0XNF7 | AFUB_014990 | Ags1/Ags2/Ags3 | |
| Alpha-1,3-glucan synthase, putative | B0XX26 | AFUB_047490 | Ags1/Ags2/Ags3 | |
| 1,3-Beta-glucan synthase catalytic subunit FksP | B0Y8S7 | AFUB_078400 | Fksp/Fks1 | |
| Cell wall protein PhiA | B0Y004 | AFUB_045170 | Aspf34 | |
| Cell wall biogenesis protein phosphatase Ssd1, putative | B0XQR1 | AFUB_010850 | Ssd1 | |
| Cell wall protein, putative | B0XXP9 | AFUB_038170 | MidA | |
| GPI-anchored cell wall protein, putative | B0Y688 | AFUB_066060 | ||
| GPI-anchored cell wall organization protein Ecm33 | B0Y5M3 | AFUB_063890 | Ecm33 | |
| GPI-anchored protein, putative | B0YDG5 | AFUB_095500 | ||
| Cell wall integrity signaling protein Lsp1, putative | B0Y7E0 | AFUB_073480 | Pil1 | |
| Cell wall serine-threonine-rich galactomannoprotein Mp1 | B0YEP2 | AFUB_099880 | Mp1 | |
| 1,3-Beta-glucanosyltransferase Gel1 | B0XT72 | AFUB_018250 | Gel1 | |
| 1,3-Beta-glucanosyltransferase Gel4 | B0XVI5 | AFUB_022370 | Gel4 | |
| Mannan endo-1,6-alpha-mannosidase | B0XXF1 | AFUB_048110 | ||
| Alpha-1,6-mannosyltransferase subunit (Mnn9), putative | B0XTG8 | AFUB_018530 | Mnn9 | |
| Dolichol-phosphate mannosyltransferase, putative | B0XXW0 | AFUB_038750 | ||
| Protein mannosyltransferase 1 | B0XYZ3 | AFUB_042600 | Pmt1 | |
| Alpha-1,2-mannosyltransferase (Kre2), putative | B0Y0S4 | AFUB_058360 | Ktr1 | |
| Alpha-1,2-mannosyltransferase (Kre5), putative | B0Y1C4 | AFUB_059750 | Ktr7 | |
| Alpha-1,2-mannosyltransferase (Ktr4), putative | B0Y2F5 | AFUB_051270 | Ktr4 | |
| Alpha-1,6-mannosyltransferase subunit, putative | B0Y6R0 | AFUB_067830 | Mnn11 | |
| UDP-glucose 4-epimerase | B0Y0S6 | AFUB_058380 | Uge5 | |
| UDP-glucose:glycoprotein glucosyltransferase, putative | B0XTX7 | AFUB_019450 | ||
| Glycosyl transferase, putative | B0XYK7 | AFUB_041250 | Gt4b/Gt4c | |
| Glycosyl transferase, putative | B0YAG3 | AFUB_084570 | Och3 | |
| Glycosyl transferase, putative | B0XZM8 | AFUB_044600 | ||
| N-Glycosyl-transferase | B0Y5M8 | AFUB_063940 | ||
| Lysophospholipase 3 | B0XZV8 | AFUB_034540 | Plb3 | |
| Lysophospholipase 1 | B0Y665 | AFUB_065820 | Plb1 | |
| Rho GTPase Rho1 | B0Y776 | AFUB_072830 | Rho1 | |
| 1,3-Beta-glucanosyltransferase Bgt1 | B0XQR5 | AFUB_010890 | Bgt1 | |
| Cell wall glycosidase | B0XNL0 | AFUB_015530 | Aspf9/Crf1 | |
| Cell wall glucanase, putative | B0XY72 | AFUB_039870 | Crh3 | |
| Mannan polymerase II complex ANP1 subunit Anp1, putative | B0XUV6 | AFUB_031580 | Van1 | |
| SUN domain protein (Uth1), putative | B0YCQ5 | AFUB_091030 | Sun1 | |
| Probable beta-glucosidase BtgE | B0Y9Q9 | AFUB_081940 | BtgE/Sw11 | |
| Putative UDP-galactopyranose mutase | B0XWU8 | AFUB_036480 | Ugm1 | |
| Endo alpha-1,4 polygalactosaminidase, putative | B0XYK5 | AFUB_041230 | Ega3 | |
a
For threshold detection limits and false-discovery rates, please see Materials and Methods.