TABLE 2.
Recombinant proteins used as screening antigens and summary of results
| Species and/or protein (B31 ORF)a |
Description | % Ab-positive samples (no. of samples positive/total no.) |
Reference |
|---|---|---|---|
| B. burgdorferi | |||
| OspA (BBA15) | Surface lipoprotein essential for survival in ticks; produced in culture and in ticks but not in mammals |
6.3 (8/128) (equivocal) | 16 |
| OspB (BBA16) | Same as described above for OspA; forms an operon with ospA |
0 (0/128) | 16 |
| VlsE (BBF0041) | Surface lipoprotein; functions in immune evasion; not produced during cultivation or in ticks; expressed in infected mammals |
63.3 (81/128) | 17 |
| DbpA (BBA24) | Decorin binding protein; facilitates dissemination during early stage infection; produced in vitro and in vivo |
56.2 (72/128) | 18 |
| DbpB (BBA25) | See information for DbpA above | 68.0 (87/128) | 18 |
| OspE (BBL39) | Factor H binding protein; facilitates complement evasion; all LD spirochete strains encode multiple OspE paralogs |
15.6 (20/128) | 19 |
| OspE (BBN38) | See information for BBL39 above | 16.4 (21/128) | 20 |
| OspF (BBR42) | Expression upregulated in mammals; Ab to OspF has been suggested to be a marker for chronic infection; most strains produce multiple OspF paralogs |
43.0 (55/128) | 21 |
| OspF (BBM38) | See information for BBR42 above | 40.6 (52/128) | 21 |
| OspF (BBO39) | See information for BBR42 above | 48.4 (62/128) | 21 |
| Mlp (BBA36) | Surface lipoprotein; expression upregulated in mammals; Mlp protein family member |
51.6 (66/128) | 22 |
| Uncharacterized protein (BBK53) | Surface lipoprotein; uncharacterized | 18.8 (24/128) | |
| P35 (BBA73) | Function unknown; surface protein; member of paralogous protein family 54; upregulated in mammals |
48.4 (62/128) | 23 |
| Protein of unknown function (BB0238) |
Function unknown; required for infection in mammals | 27.3 (35/128) | 24 |
| A. phagocytophilum | |||
| P130 | Unique to A. phagocytophilum; localizes to the vacuolar membrane | 60.9 (78/128) | 10 |
| P44 | Porin protein involved in immune evasion; homologs are found in A. platys and A. marginale |
72.7 (93/128) | 9 |
| Aph_1235 | Specific to the infectious dense core cell | 3.3 (3/90) | 25 |
| Asp14 | Adhesin; homologs are found in some Anaplasma and Ehrlichia species |
7.8 (7/90) | 26 |
| OmpA | Adhesin; homologs are found in some Anaplasma and Ehrlichia species |
18.9 (17/90) | 26 |
| FhbB | T. denticola FH binding protein; negative control | 0 (0/128) | 13 |
a
The ORF designations listed are those assigned to B. burgdorferi strain B31.