FIG 4.
Growth properties of sod3Δ/Δ and overexpressing (sod3Δ/Δ::SOD3i+) or (sod3Δ/Δ::SOD3i−) isoforms. (A) (Panel 1) Growth curves of sod3Δ/Δ knockout and individual isoform (sod3Δ/Δ::SOD3i+ or sod3Δ/Δ::SOD3i−) overexpressing strains in YPD and AMB (0.2 μg/ml) in the presence and absence of doxycycline (DOX; 50 μg/ml). The DOX-only condition was also used as a control. (Panel 2) Strains in YPD and MND (0.1 mM) in the presence and absence of doxycycline (DOX; 50 μg/ml). (B) The susceptibility of sod3Δ/Δ and the individual isoform (sod3Δ/Δ::SOD3i+ or sod3Δ/Δ::SOD3i−) overexpressing strains to AMB and MND was tested by spot assays on solid agar medium under similar conditions as in panel A, confirming the growth curve results.