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. 2020 Aug 13;8(4):e00638. doi: 10.1002/prp2.638

FIGURE 3.

FIGURE 3

Target gene expression following the concomitant activation of NRF2 and LPS‐activating signals in peritoneal macrophages. The expression of NRF2 and LPS target genes was evaluated in peritoneal macrophages treated with vehicle (veh, white bars), tBHQ (grey bars), LPS (black bars), and the combination of the two stimuli (dashed grey bars) for 3 or 16 h, as indicated. Real‐time PCR was used to analyze the mRNA levels coding (A) HMOX1 (B, C) TALDO1, TKT IDH1, IRG1, and PPAT, and (D) KI67. Data sets for each gene were calculated using the 2−ΔΔCt method and expressed in relation to 3 h veh samples. Data are presented as mean values ± SEM (n = 3) of a single experiment representative of at least two other independent experiments. Results were analyzed by one‐way ANOVA followed by Bonferroni's multiple comparisons test. (df = 11; HMOX1, 3 h: F = 0.7, 16 h = 1.074; TALDO1, 3 h: F = 8.255, 16 h: F = 869.2; TKT, 3 h: F = 1.114, 16 h: F = 188.7; IDH1, 3 h: F = 93.44, 16 h: F = 353.1; IRG1, 3 h: F = 21.25, 16 h: F = 803.1; PPAT, 3 h: F = 12.73, 16 h: F = 5.013; KI67 3 h: F = 374.2, 16 h: F = 100.6). (*) P < .05, (**) P < .01, (***) P < .001 vs 6 h veh; (###) P < .001 vs tBHQ; ($$$) P < .001 vs LPS