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. Author manuscript; available in PMC: 2021 Jan 20.
Published in final edited form as: Nat Plants. 2020 Jul 20;6(8):957–969. doi: 10.1038/s41477-020-0726-z

Extended Data Fig. 4. Arabidopsis TREX-2 components and sac3 mutant alleles.

Extended Data Fig. 4

(a) Yeast two-hybrid assays to test interactions among TREX-2 components (THP1, SAC3A, SAC3B, DSSI-(I), DSSI-(V), CEN1, and CEN2). The empty vectors AD and BD were included as negative controls. After mating, diploid yeast cells containing both the bait and prey plasmids grew on SD-Trp/-Leu (DDO) medium, and diploid yeast cells in which the bait and prey proteins interacted grew into blue colonies on SD-Trp/-Leu/-His/-Ade+X-α-gal Aureobasidin A (QDO/X/A) medium. The experiment was repeated two times with similar results. (b) Diagrams of SAC3A, B, and C genes showing the different mutant alleles. Triangles indicate positions of T-DNA insertions. Black arrows indicate primers used for RT-PCR. Lines below the gene models indicate conserved protein domains in homologous proteins. (c) RT-PCR analyses of SAC3 gene expression in different sac3 mutants. The primers used are shown as black arrows in (b). The experiment was repeated two times with similar results.