Table 1.
Biochemical evaluations at basal and stimulated conditions
| Time | Cortisol R1 (nmol/L) | Cortisol R2 (nmol/L) | Cortisol LC-MS/MS (nmol/L) | p value (R1 vs. R2 and LC-MS/MS) | |
|---|---|---|---|---|---|
|
LDCT N = 15 |
0′ | 306 (193) | 212 (102) | 229 (116) | < 0.001 |
| 30′ | 599 (315) | 414 (163) | 436 (199) | < 0.001 | |
| 60′ | 458 (386) | 317 (229) | 378 (215) | < 0.001 | |
|
SDCT N = 15 |
0′ | 516 (348) | 348 (190) | 359 (182) | < 0.001 |
| 30′ | 789 (190) | 538 (124) | 552 (119) | < 0.001 | |
| 60 | 927 (163) | 635 (116) | 646 (168) | < 0.001 |
LDCT, low-dose (1 μg) ACTH test; SDCT, standard dose (250 μg) ACTH test; R1, Roche I; R2, Roche II cortisol assay; LC-MS/MS, liquid chromatography tandem mass spectrometry; AI, adrenal insufficiency. Cortisol levels are expressed as median (interquartile range); categorical variables are expressed as number (percentage). Conventional unit conversion factors, SI cortisol levels/27.6