Table 3.

Clinical and biochemical characteristics of patients diagnosed as AI by R2 but not by R1

Patient	Age	Sex	Diagnosis	Test type	Ongoing GC replacement	R1 (nmol/L)			R2 (nmol/L)			TM (nmol/L)
						0′	30′	60′	0′	30′	60′	0′	30′	60′
P1	76	M	Unilateral adrenalectomy for subclinical hypercortisolism by adrenal adenoma	SDCT	Yes	516	585	596	403	417	442	425	477	483
P2	61	M	GH-deficiency + hypogonadism + hypothyroidism following pituitary surgery	LDCT	No	425	704	657	259	453	419	276	508	444
P3	40	F	Microprolactinoma	LDCT	No	248	599	475	201	414	359	218	436	384
P4	75	M	GH-deficiency + hypogonadism following pituitary surgery	LDCT	No	306	638	577	207	464	386	229	472	400
P5	50	F	Microprolactinoma	LDCT	No	268	541	395	212	378	293	174	223	320
P6	65	F	Non-classical congenital adrenal hyperplasia	SDCT	No	546	679	693	326	295	353	337	408	373
P7	55	F	Partial empty sella	LDCT	No	389	690	458	237	461	317	271	527	378

AI, adrenal insufficiency; LDCT, low-dose (1 μg) ACTH test; SDCT, standard dose (250 μg) ACTH test; GC, glucocorticoids

R1, Roche I cortisol assay; R2, Roche II cortisol assay; LC-MS/MS, liquid chromatography tandem mass spectrometry. *p < 0.001 for R1 vs. both R2 and LC-MS/MS cortisol concentrations. Conventional unit conversion factors, SI cortisol levels/27.6