Fig. 5.

SRSF2 associates with the P300/CBP complex. a Protein levels of H3K27Ac, Histone H3 and β-actin in Jurkate E6 cells transfected with SRSF2 siRNAs or negative control siRNAs were measured by western blotting. b Jurkate E6 cells were cultured in poly-d-lysine,coated plates, fixed, incubated with an anti-SRSF2 antibody (red), anti-P300 antibody (green), or anti-CBP antibody (green), and subjected to a confocal analysis. The intensity plots of the red and green channels were analyzed with ImageJ software. Scale bars, 10 µm. c Jurkate E6 cells lysates were collected and immuno-precipitated with an anti-SRSF2 antibody or anti-IgG antibody, followed by a western blot analysis of SRSF2, P300, and CBP