Fig. 6.

SRSF2 associates with STAT3 and H3K27Ac. a. Jurkate E6 cells transfected with SRSF2 siRNAs or negative control siRNAs were cultured in poly-d-lysine-coated plates, fixed, incubated with an anti-STAT3 Y705p antibody (red), and anti-H3K27Ac antibody (green), and subjected to a confocal analysis. The intensity plots of the red and green channels were analyzed with ImageJ software. Scale bars, 10 μm. b. Jurkate E6 cell lysates transfected with SRSF2 siRNAs or negative control siRNAs were collected and immuno-precipitated with an anti-STAT3 Y705p antibody or anti-IgG antibody, followed by a western blot analysis of STAT3 Y705p, H3K27Ac, Histone H3, and β-actin. (C). Jurkate E6 cells were cultured in poly-d-lysine-coated plates, fixed, incubated with an anti-SRSF2 antibody (red), anti-H3K27Ac antibody (green) or anti-STAT3 Y705p antibody (green), and subjected to a confocal analysis. The intensity plots of the red and green channels were analyzed with ImageJ software. Scale bars, 10 μm. d. Jurkate E6 cell lysates were collected and immuno-precipitated with an anti-SRSF2 antibody or anti-IgG antibody, followed by a western blot analysis of H3K27Ac and STAT3 Y705p. e. Schematic model of the roles of SRSF2 in P300/CBP-mediated H3K27 acetylation ① and STAT3 Y705p-mediated gene transcription ②