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. 2020 Aug 13;10:13713. doi: 10.1038/s41598-020-70530-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design of a representative stacked multiple promoter:recombinant gene expression system developed for sugarcane. Promoter 1, 2 and 3 combinations can be any combination of the constitutive promoters maize, ubiquitin 1, sugarcane proline rich protein and sugarcane elongation factor 1α or the culm-regulated promoters from sugarcane dirigent16 and Sugarcane bacilliform virus. Vector assembly and cloning sites are indicated under “Materials and methods” section. BvLz_m, maize codon-optimized bovine lysozyme gene; 35ST, terminator derived from Cauliflower mosaic virus 35S RNA; NOST, Agrobacterium tumefaciens nopaline synthase terminator; 3′UTR, 3′ untranslated region of Sorghum mosaic virus.