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. 2020 Aug 13;10:13713. doi: 10.1038/s41598-020-70530-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Enhancement of culm biomass and yield of recombinant bovine lysozyme (BvLz_m) by fertilization in triple promoter:BvLz_m sugarcane lines. BvLz_m activity of four representative lines is shown as determined by enzyme-linked immunosorbent assay in juice extract of culms (1.0 kg of culm). Values represented four biological samples and three technical replications at 2, 6 and 8 months following low (LF) or high (HF) fertilization. Values with different letters are significantly different (p < 0.05). BvLz_m, maize codon-optimized BvLz; 32C, pUPE:BvLz_m line; 18, 44 and 54, pUDE:BvLz_m lines; U, maize ubiquitin 1 promoter; P, sugarcane proline-rich protein promoter; E, sugarcane elongation factor 1α promoter; and D, sugarcane dirigent16 promoter.