. 2020 Aug 13;10:13713. doi: 10.1038/s41598-020-70530-z

Table 4.

Leaf nutrient contents in leaves of triple promoter:bovine lysozyme expressing sugarcane lines grown under two fertilization levels.

Line/treatment	Macronutrient content (mg/g tissue dry mass)
Line/treatment	Nitrogen	Phosphorus	Potassium	Magnesium
pUPE:BvLz_m 32C
LF	8.8±1.0^a	1.5 ± 0.2^a	11.7 ± 0.2^a	1.4 ± 0.4^a
HF	15.0 ± 1.0^b	3.1 ± 0.4^b	19.5 ± 0.2^b	3.1 ± 0.3^b
pUDE:BvLz_m 18
LF	10.3 ± 0.1^a	2.2 ± 0.2^a	17.3 ± 0.3^a	1.1 ± 0.1^a
HF	18.7 ± 2.0^b	4.0 ± 0.2^b	20.0 ± 1.8^b	2.1 ± 0.2^b
pUDE:BvLz_m 19
LF	12.4 ± 2.0^a	2.1 ± 0.2^a	17.3 ± 0.5^a	1.1 ± 0.1^a
HF	16.8 ± 1.0^b	3.3 ± 0.3^b	19.5 ± 0.7^b	3.0 ± 0.2^b
Non-transformed
LF	9.6 ± 1.5^a	1.7 ± 0.2^a	13.0 ± 0.4^a	1.8 ± 0.2^a
HF	15.0 ± 2.1^b	2.9 ± 0.4^b	15.2 ± 1.4^b	3.1 ± 0.3^b

Leaf tissue was sampled from 8-month-old plants of three representative lines. Values represent means from three biological samples ± standard errors. Means are compared column-wise. Values with different letters are significantly different (p < 0.0001). BvLz_m: maize codon-optimized bovine lysozyme (BvLz); pUPE:BvLz_m: BvLz_m expressed from three promoters, maize ubiquitin 1, sugarcane proline-rich protein and sugarcane elongation factor 1α (pSHEF1α); pUDE:BvLz_m: BvLz_m expressed from three promoters, maize ubiquitin 1, sugarcane dirigent16 and pSHEF1α; HF: high fertilization rate; LF: low fertilization rate.