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. 2020 Aug 13;10:13757. doi: 10.1038/s41598-020-70735-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Heterozygous mtnr1a VIL mutant young adult (juvenile) frogs display normal rods and cones. (a) WT juvenile frogs display normal rods (ROS) and cones. (b) F1 mtnr1a VIL heterozygous mutant juvenile sibling frogs display healthy-looking rods and cones, although the ROS density appears to be lower than in WT (asterisks). Mtnr1a 56-bp deletion mutant tadpoles do not display rod dystrophy. (c) Four-month-old WT tadpole retinas and (d) F1 mtnr1a heterozygous 56-bp deletion (potential knockout) siblings exhibit healthy-appearing rods (ROS) and cones (white arrows). RPE retinal pigment epithelium, ROS rod outer segments, ONL outer nuclear layer, INL inner nuclear layer. Magnification bars = 20 µm.