Table 1.

Physiochemical properties of ChimeraT and the individual T-cell epitopes.

Protein/peptide	Amino acid sequence	Number of amino acids	Molecular weight (kDa)	Isoelectric point	Instability index	Aliphatic index	GRAVY
ChimeraT	YIMSGPARYVYFHMVLPVEAQGGRLEDQAPSTHNVEVPFVKTFTYSVLDIQPNEGGNYDPNVWCAVPNCITCDRLDPSNRGGVRVLYQPNVENLYHIYRHIGVNAETVL	109	12.31	5.40	51.03	84.77	−0.245
LiHyp1 epitope	YIMSGPARYVYFHMVLPVEAQ	21	2.47	6.75	50.78	88.10	0.386
EIF5a epitope	RLEDQAPSTHNVEVPFVKTFTYSVLDIQPNE	31	3.54	4.50	57.65	78.39	−0.535
LiHyp2 epitope	NYDPNVWCAVPNCITCDRLDPSNR	24	2.76	4.43	39.18	60.83	−0.667
PHB epitope	VRVLYQPNVENLYHIYRHIGVNAETVL	27	3.21	6.90	54.73	129.63	0.007

GRAVY grand average of hydropathy.

ChimeraT was constructed using amino acid sequences containing selected CD4⁺ and CD8⁺ T-cell epitopes from Prohibitin (PHB; Amino Acids 104–131), EIF5a (Amino Acids 71–101), LiHyp1 (Amino Acids 163–183) and LiHyp2 (Amino Acids 416–439) proteins.