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. 2020 Aug 13;11:4052. doi: 10.1038/s41467-020-17783-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Chemiluminescence spectrum of ADLumin-1 in DMSO; b Quantitative analysis of chemiluminescence intensity of ADLumin-1 in DMSO solutions before and after stirring. Representative images were inserted. c Quantification of chemiluminescence intensity of ADLumin-1 with (red) or without (black) bubbled oxygen; d LC-MS spectra of ADLumin-1 and ADLumin-3 (the oxidation product). Note: ADLumin-1 (t = 3.05 min), ADLumin-3 (t = 3.85 min); e Proposed oxygen-dependent mechanism of ADLumin-1 for chemiluminescence generation. Data are represented as mean ± s.d. with n = 3 biologically independent samples. P = 0.0009 (b). The P-values were generated by Graphpad Prism 8 with two-tailed unpaired t-test; ***P-value < 0.001; Source data underlying b and c are provided as Source Data file.