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. 2020 Aug 13;11:4052. doi: 10.1038/s41467-020-17783-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a In vivo brain imaging of WT and 5XFAD mice with mixture of ADLumin-1 and CRANAD-3; b In vivo emission spectra of CRET pair (red line-5xFAD) and control (black line-WT), and ADLumin-1 only (pink line-5xFAD) and ADLumin-1 only control (blue line-WT); c–e Quantitative analysis of the images with CRET pair with the setting of open filter (c), at 640 nm (d) and after spectral unmixing (e). Data are represented as mean ± s.d. with n = 3 biologically independent animals. P = 0.0059 (c), P = 0.0292 (d), P = 0.0133 (e). The P-values were generated by Graphpad Prism 8 with two-tailed unpaired t-test; *P-value < 0.05, **P-value < 0.01. Source data underlying c–e are provided as Source Data file.