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. 2020 Aug 7;11:918. doi: 10.3389/fphys.2020.00918

FIGURE 4.

FIGURE 4

Down-regulation of proliferation, odontogenic differentiation and β-catenin in response to the knockdown of Rspo2. The knockdown efficiency of Rspo2 was verified by qRT-PCR and western blotting (A–C). EdU (D,E) and CCK-8 assay (F) indicated a reduced proliferation of shRspo2 cells compared with the control. The mRNA and protein expression of DSPP, DMP-1, ALP, and BSP of shRspo2 and shNC cells following 10 days of odontogenic induction (G–I). A rescue experiment was performed by adding 50 or 200 ng/mL rhRspo2 to treat shRspo2 cells (J–L). The expression and localization of the β-catenin of shRspo2 cells and shNC cells was detected by immunofluorescence (M) and western blotting (N,O). The experiments were performed for three times. *p < 0.05, and **p < 0.01 compared with the control group.