FIGURE 5.

Blockade of Wnt signaling with DKK-1 can inhibit Rspo2-induced activation of Wnt/β-catenin signaling and cell differentiation. The mRNA and protein expression of DSPP, DMP-1, ALP, and BSP of cells treated with or without rhRspo2 (200 ng/mL) or DKK-1 (800 ng/mL) after 10 days of odontogenic induction (A–C). The expression (D,E) and localization (F) of β-catenin in cells treated with or without rhRspo2 (200 ng/mL) or DKK-1 (800 ng/mL) for 2 h. The experiments were performed for three times. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group.