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. 2020 Aug 7;11:918. doi: 10.3389/fphys.2020.00918

TABLE 1.

Primers used for the analysis of mRNA levels by qRT-PCR.

Gene Forward primer Reverse primer
DSPP 5′-CTGTTGGGAAGAGCCAAGATAAG-3′ 5′-CCAAGATCATTCCATGTTGTCCT-3′
DMP-1 5′-GTGAGTGAGTCCAGGGGAGATAA3’ 5′-TTTTGAGTGGGAGAGTGTGTGC-3′
ALP 5′-TAAGGACATCGCCTACCAGCTC-3′ 5′-TCTTCCAGGTGTCAACGAGGT-3′
BSP 5′-GATTTCCAGTTCAGGGCAGTAG-3′ 5′-CCCAGTGTTGTACAGAAAGTG-3′
GAPDH 5′-CTTTGGTATCGTGGAAGGACTC-3′ 5′-GTAGAGGCAGGGATGATGTTCT-3′
LGR4 5′-CGGGCAACGACCTTTCTTT-3′ 5′-CACAGATGCCGTAACTGAACAA-3′
LGR5 5′-TCCCTGCGTCTGGATGCTAA-3′ 5′-CCAGGGAGTGGATTCTATTGTTATG-3′
LGR6 5′-TGCCTTATGCCTACCAGTGCT-3′ 5′-CAGGTCCTGGTCATAGTGGTTCT-3′
ZNRF3 5′-CCAGAATTGGACCCGAAACC-3′ 5′-TCGAGCCACTTTCTGCTTGTTG-3′
LRP5 5′-TGCTCCCACATCTGTATTGCC-3′ 5′-CTCCTCGTCGCTCTGGTCAT-3′
LRP6 5′-CTTGCAGCCTGTGGGACTTAC-3′ 5′-ATGTGAACAGCCACCATTATCC-3′
Frizzled4 5′-TCCCACCACAGAACGACCAC-3′ 5′-AAGCCAGCATCATAGCCACACT-3′