Fig. 3.

VCP elicits a distinct stress-associated transcriptomic alteration in VCP TG mouse hearts vs WT mice at the end of 5W TAC. A. VCP protein levels normalized to GAPDH. n = 4 mice/group. B–C. Profiles of RNA-seq analysis. Principal component analysis (PCA) (B) and Hierarchical clustering (C) of differentially expressed genes (DEGs) with FDR < 0.05. D. The volcano plot of DEGs between the two groups. Red and green dots represent up- and down-regulated in VCP TG group. E. GO functional analysis of DEGs based on based on the FC > 2 and p < 0.05 between WT and VCP TG mice at the end of 5 W TAC, in terms of the cellular components (CC), molecular function (MF) and biological process (BP) according to p-value. N = 3–4 mice/group for B-E. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)