Figure 4.

The effect of GSK-3β inhibitor on the transcription of proinflammatory cytokines in GO fibroblasts in comparison to control fibroblasts. (A) Cells obtained from GO patients (n = 3) and control patients (n = 3) were treated with DMSO for 3 hours, as well as 10 ng/mL of IL-1β for 3 hours and IL-1β for 3 hours after pretreatment with GSK-3β inhibitor (10-µM CHIR 99021) for 1 hour. The RNA extracted from each sample was reverse-transcribed by real-time PCR and quantified. The results showed elevated levels of IL-6, IL-8, ICAM-1, COX-2, and MCP-1 in response to IL-1β treatment, which was significantly suppressed by the addition of CHIR 99021. (B) The same experiment was repeated with TNF-α. The results are shown as mean ± SD fold expression of three separate experiments (^*P < 0.05 vs. IL-1β- or TNF-α-stimulated cells).