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. 2020 Aug 13;40(8):BSR20193287. doi: 10.1042/BSR20193287

Figure 3. MiR-509-3p is expressed lower in PCa cells and inversely correlated with HOXA-AS2.

Figure 3

(A) MiR-509-3p expression in PCa tissues and adjacent normal samples were tested by qRT-PCR. (B) The correlation between HOXA-AS2 and miR-509-3p in PCa tissues was examined by Pearson’s correlation analysis. (C) The binding sites entre HOXA-AS2 and miR-509-3p were obtained from starBase database. (D) The luciferase reporter assay was implemented to attest the binding relationship entre HOXA-AS2 and miR-509-3p in LNCaP and DU145 cells. (E) RIP assay was utilized to prove the coexistence of HOXA-AS2 and miR-509-3p in RISC. (F) qRT-PCR was performed to determine miR-509-3p expression in the condition of HOXA-AS2 depletion. All data were expressed as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001.

RISC: RNA-induced silencing complex.