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. 2020 Aug 7;8:932. doi: 10.3389/fbioe.2020.00932

FIGURE 1.

FIGURE 1

YAP/TAZ signaling pathway was downregulated in cells cultured on spinach leaf decellularized scaffolds. (A) Immunofluorescence images of YAP/TAZ and nuclei (DAPI) in PC3 and SK-MEL-28 cells seeded on coverslip and spinach scaffold for 3 days. Scale bars = 15 μm. Graphs indicate the percentage of cells with nuclear YAP/TAZ (n = 3, *p < 0.05; Student t-test). (B) Quantitative RT-PCR analysis in PC3 and SK-MEL-28 cells to measure YAP and TAZ mRNA levels. Cells were grown on TCPS or spinach scaffold for 3 days. Data were normalized to expression on TCPS and indicated as mean ± SEM (n = 3, *p < 0.05; Student t-test). (C) Immunoblotting of YAP and TAZ in PC3 and SK-MEL-28 cells seeded on TCPS and spinach scaffolds. Bands intensities were quantified using ImageJ and normalized with GAPDH. Numbers represent the expression level compared to TCPS of three independent experiments. (D) Quantitative RT-PCR for YAP/TAZ target genes (CTGF and ANKRD1) in PC3 and SK-MEL-28 cells. Cells were grown on TCPS or spinach scaffold for 3 days. Data were normalized to expression on TCPS and indicated as mean ± SEM (n = 3, *p < 0.05; Student t-test).