Table 1.

Primer sequence used for RT-qPCR.

Primers name	Primer sequence	Size (bp)
Npy-F	5′- AATCAGTGTCTCAGGGCTGGAT-3′	74
Npy-R	5′-CCGCTCTGCGACACTACATC-3′
Agrp-F	5′-CGGAGGTGCTAGATCCACAGA-3′	69
Agrp-R	5′-AGGACTCGTGCAGCCTTACAC-3′
Pomc-F	5′-AAGAGCAGTGACTAAGAGAGGCCA-3′	159
Pomc-R	5′-ACATCTATGGAGGTCTGAAGCAGG-3′
Cart-F	5′-GCGCTATGTTGCAGATCGAA-3′	105
Cart-R	5′-CGTCACACATGGGGACTTGG-3′
Crh-F	5′-TCAGAGCCCAAGTACGTT-3′	113
Crh-R	5′-AGGGACTTCTCTCAGGAT-3′
Orexin-F	5′-GACGGCCTCAGACTTCTTGG-3′	118
Orexin-R	5′-GGCCCAGGGAACCTTTGTAG-3′
Mch-F	5′-TCCAATGCACTCTTGTTTGGC-3′	310
Mch-R	5′-TGTTTGGAGCCTGTGTTCTTTG-3′
36B4-F	5′-CGACCTGGAAGTCCAACTAC-3′	109
36B4-R	5′-ATCTGCTGCATCTGCTTG-3′

F, forward; R, reverse.