Skip to main content
. 2020 Aug 13;10:13684. doi: 10.1038/s41598-020-70291-9

Figure 2.

Figure 2

The effects of labelling on UCMSCs (A) Cell viability as determined by the Trypan blue exclusion assay. Viability is calculated as follows: percent viable cells = 100 × (number of viable cells per mL of aliquot)/(total number of cells per mL of aliquot). No significant impairment is observed. (B) Cell proliferation, as evaluated by the MTT assay (MTT: 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide). Proliferation as a percent is calculated as follows: 100 × (optical density of “treated” well − blank)/(optical density of “control” well − blank) (i.e., the optical density measurements are scaled to those of the untreated control cells). A significant impairment is observed at 100 μg/mL of USPIO. *P < 0.05 compared with control. UCMSCs umbilical cord-derived mesenchymal stem cells, SPIO superparamagnetic iron oxide, USPIO ultrasmall superparamagnetic iron oxide.